Journal: Scientific Reports

Article Title: Curcumin preserves bone health compromised by diabetes by inhibiting osteoporosis through regulation of the SIRT3/FoxO3a signalling pathway

doi: 10.1038/s41598-025-15165-8

Figure Lengend Snippet: Curcumin upregulates the expression of the oxidative stress-related proteins Nrf2 and SIRT3 in bone tissue cells. Histograms indicate the SIRT3 protein expression levels relative to the control group. Values are expressed as mean ± standard deviation for three independent experiments ( n = 3). * p < 0.05, *** p < 0.001 vs. control group, # p < 0.05, ## p < 0.01 vs. HG group.

Article Snippet: Curcumin (Solarbio), mouse anti-SIRT3 antibody (Proteintech) , mouse anti-FoxO3a antibody (Proteintech), mouse anti-cytochrome c antibody (Proteintech), mouse anti-NRF2 antibody (Proteintech), mouse anti-NOX2 antibody (Proteintech), mouse anti-caspase 3 antibody (Proteintech), mouse anti-SOD2 antibody (Proteintech), rabbit anti-mouse SIRT3 antibody (Proteintech) Proteintech), rabbit anti-mouse Bcl2 antibody (Proteintech), mouse anti-β-actin antibody (Beijing Zhongyi Jinqiao Company), sodium pentobarbital (Sigma), paraformaldehyde (Xi’an Reagent Factory), α-D-glucose (Sigma), anti-osteocalcin (OCN) antibody (Merck & Millipore ), NQO1 (Proteintech), mouse osteoprotegerin (OPG) antibody (R&D), minimum essential medium alpha (α-MEM) (Bioind), dimethyl sulfoxide Hybri-Max (Sigma), Flow Cytometry Kit (Jiangsu Biyuntian), osteogenic induction complete medium (Pythonbio), Mitochondrial Membrane Potential Assay Kit (Jiangsu Biyuntian), ROS Reactive Oxygen Species Assay Kit (Jiangsu Biyuntian).

Techniques: Expressing, Control, Standard Deviation

Journal: Science Advances

Article Title: Targeting HMGB2 acts as dual immunomodulator by bolstering CD8 + T cell function and inhibiting tumor growth in hepatocellular carcinoma

doi: 10.1126/sciadv.ads8597

Figure Lengend Snippet: ( A ) Location of differential accessible ATAC-seq peaks in NC and Hmgb2 -cKO CD8 + T cells. 3′UTR, 3′ untranslated region; 5′UTR, 5′ untranslated region. ( B ) Chromatin accessibility changes of genes associated with effector function and mitochondrial transcription factors. ( C ) The correlation between Hmgb2 , Tfam , and Tfb1m expression in CD8 + T cells from murine scRNA-seq data. ( D ) mRNA levels of Keap1 and Nfe2l2 in NC and Hmgb2 -cKO CD8 + T cells ( n = 6). ( E ) mRNA levels of ARE genes in NC and Hmgb2 -cKO CD8 + T cells ( n = 3). ( F ) Protein changes of KEAP1 and NRF2 after Hmgb2 knockout. ( G ) The ubiquitination of NRF2 after Hmgb2 knockout. IB, immunoblot. ( H ) Protein changes of KEAP1 and NRF2 after stimulation of mouse recombinant HMGB2 protein and IN-1. NC CD8 + T cells were treated with recombinant HMGB2 protein (500 ng/ml) and/or IN-1 (10 μM) for 48 hours. ( I ) The ubiquitination of NRF2 after cell treatment as in (H). ( J ) Immunofluorescence staining of spontaneous HCC tissues in NC and Hmgb2 -cKO mice. NRF2 + KEAP1 − CD8 + T cells were labeled. Scale bars, 20 μm (left) and 10 μm (right). HP, high power field. ( K ) Seahorse extracellular flux analysis of OCR in different CD8 + T cell groups ( n = 6 to 8) after cell treatment as in (H). ( L ) Quantification of seahorse extracellular flux analysis of OCR of different CD8 + T cells as in (K). Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Pearson test for (C) Student’s t test for (D), (E), and (J). Two-way ANOVA test for (L).

Article Snippet: Anti-human/mouse NRF2 , 16396-1-AP , Proteintech, China.

Techniques: Expressing, Knock-Out, Ubiquitin Proteomics, Western Blot, Recombinant, Immunofluorescence, Staining, Labeling

Journal: Science Advances

Article Title: Targeting HMGB2 acts as dual immunomodulator by bolstering CD8 + T cell function and inhibiting tumor growth in hepatocellular carcinoma

doi: 10.1126/sciadv.ads8597

Figure Lengend Snippet: Markers and article numbers of antibodies. PE, phycoerythrin; FITC, fluorescein isothiocyanate; APC, antigen-presenting cell; HRP, horseradish peroxidase; mAb, monoclonal antibody.

Article Snippet: Anti-human/mouse NRF2 , 16396-1-AP , Proteintech, China.

Techniques: Ubiquitin Proteomics, Purification, In Vivo

Journal: Frontiers in Bioscience-Landmark

Article Title: VSIG4 Alleviates Intracranial Hemorrhage Injury by Regulating Oxidative Stress and Neuroinflammation in Macrophages via the NRF2/HO-1 Signaling Pathway

doi: 10.31083/fbl37810

Figure Lengend Snippet: Fig. 2. Recombinant VSIG4 activates the NRF2 signaling pathway to inhibit oxidative stress and inflammatory responses in

Article Snippet: Subsequently, the membrane was incubated overnight at 4 °C with primary antibodies, namely rabbit anti-mouse NRF2 (1:2000; CST, 12721, Danvers, MA, USA), heme oxygenase-1 (HO-1) (1:2000; CST, 70081, USA), B-cell lymphoma 2 (Bcl-2) (1:2000; CST, 3498, USA), Bax (1:2000; CST, 2772, USA), VSIG4 (1:2000; Abcam, ab252933, Cambridge, UK), and GAPDH (1:2000; CST, 2118, USA).

Techniques: Recombinant

Journal: Frontiers in Bioscience-Landmark

Article Title: VSIG4 Alleviates Intracranial Hemorrhage Injury by Regulating Oxidative Stress and Neuroinflammation in Macrophages via the NRF2/HO-1 Signaling Pathway

doi: 10.31083/fbl37810

Figure Lengend Snippet: Fig. 5. The NRF2 inhibitor Brusatol inhibits the protective effect of VSIG4 in vitro. (A–C) Western blotting analysis of NRF2 and HO-1 in RAW264.7 cells exposed to Brusatol, n = 3. (D–F) Levels of GSH, SOD and MDA levels in Brusatol-exposed RAW264.7

Article Snippet: Subsequently, the membrane was incubated overnight at 4 °C with primary antibodies, namely rabbit anti-mouse NRF2 (1:2000; CST, 12721, Danvers, MA, USA), heme oxygenase-1 (HO-1) (1:2000; CST, 70081, USA), B-cell lymphoma 2 (Bcl-2) (1:2000; CST, 3498, USA), Bax (1:2000; CST, 2772, USA), VSIG4 (1:2000; Abcam, ab252933, Cambridge, UK), and GAPDH (1:2000; CST, 2118, USA).

Techniques: In Vitro, Western Blot

Journal: Frontiers in Bioscience-Landmark

Article Title: VSIG4 Alleviates Intracranial Hemorrhage Injury by Regulating Oxidative Stress and Neuroinflammation in Macrophages via the NRF2/HO-1 Signaling Pathway

doi: 10.31083/fbl37810

Figure Lengend Snippet: Fig. 6. The NRF2 inhibitor Brusatol inhibits the protective effect of VSIG4 in vivo. (A) Neurological scores, n = 5. (B) Brain water content, n = 5. (C–G) Western blot analysis was conducted to measure the protein levels of NRF2, HO-1, Bcl-2, and Bax in brain tissues

Article Snippet: Subsequently, the membrane was incubated overnight at 4 °C with primary antibodies, namely rabbit anti-mouse NRF2 (1:2000; CST, 12721, Danvers, MA, USA), heme oxygenase-1 (HO-1) (1:2000; CST, 70081, USA), B-cell lymphoma 2 (Bcl-2) (1:2000; CST, 3498, USA), Bax (1:2000; CST, 2772, USA), VSIG4 (1:2000; Abcam, ab252933, Cambridge, UK), and GAPDH (1:2000; CST, 2118, USA).

Techniques: In Vivo, Western Blot

Journal: Pharmacology research & perspectives

Article Title: Soluble Guanylate Cyclase Stimulator, BAY41-8543: A Promising Approach for the Treatment of Chronic Heart Failure Caused by Pressure and Volume Overload.

doi: 10.1002/prp2.70087

Figure Lengend Snippet: FIGURE 2 | Myocardial protein levels and representative western blot images (A) of Nrf 2 (B), and antioxidant enzyme SOD1 (C) implicated in anti-oxidant and anti-inflammatory response, as well as NfκB (D) and MPO (E) related to inflammation and oxidative damage. Proteins were measured in the left ventricle of HanSD rat, and TGR rats with ACF or without (sham) that survived until the end of the treatment with sGC stim- ulator, ACEi or their combination. Values are normalized to GAPDH, presented as mean ± SD, *p ≤ 0.05 vs. TGR; by one-way ANOVA and Tukey's multiple comparison test. Nrf2: Nuclear factor erythroid 2–related factor 2; SOD1: Superoxide dismutase 1; NfκB: Nuclear factor kappa B; MPO: Myeloperoxidase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase. Membranes for representative western blot images were cut and stripped for reprobing, which is the reason why only two GAPDH are shown.

Article Snippet: Supplier Anti- galectin- 3 Rabbit /1:1000/ #89572 Cell Signaling Technology, Danvers, MA, USA Anti- GAPDH Rabbit /1:1000/ sc- 25 778 Santa Cruz Biotechnology, Texas, USA Anti- pkcδ Rabbit /1:1000/ sc- 213 Santa Cruz Biotechnology, Texas, USA Anti- MMP2 Rabbit /1:500/ sc- 10 736 Santa Cruz Biotechnology, Texas, USA Anti- nfκb Rabbit /1:500/ sc- 372 Santa Cruz Biotechnology, Texas, USA Anti- PKG Rabbit /1:1000/ sc- 25 429 Santa Cruz Biotechnology, Texas, USA Anti- SMAD2/3 Rabbit /1:1000/ #3102 Cell Signaling Technology, Colorado, USA Anti- TGF- β1 Rabbit /1:1000/ SAB4502954 Sigma- Aldrich, Missouri, USA Anti- SOD1 Rabbit /1:500/ sc- 11 407 Santa Cruz Biotechnology, Texas, USA Anti- Keap1 Mouse /1:500/ sc- 514 914 Santa Cruz Biotechnology, Texas, USA Anti- Nrf2 Mouse /1:500/ sc- 365 949 Santa Cruz Biotechnology, Texas, USA Anti- MPO Mouse /1:500/ sc- 52 707 Santa Cruz Biotechnology, Texas, USA Anti- HSP70 Mouse /1:500/ sc- 32 239 Santa Cruz Biotechnology, Texas, USA Anti- Mouse — /1:2000/ #7076S Cell Signaling Technology, Danvers, MA, USA Anti- Rabbit — /1:2000/ #7074S Cell Signaling Technology, Danvers, MA, USA nloaded from https://bpspubs.onlinelibrary.w iley.com /doi/10.1002/prp2.70087 by N at Prov Indonesia, W iley O nline L ibrary on [07/04/2025].

Techniques: Western Blot, Comparison